Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
نویسندگان
چکیده
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β(1) domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
منابع مشابه
Analysis of polymorphism of MHC class II BuLA DRB3 exon 2 gene in North West Iranian populations of the Water buffalo (Bubalus bubalis) through PCR-SSCP
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